In Vitro Skin Testing

Efficacy and Claims Support for Pharmaceutical and Cosmeceutical Products

In order to meet our customers’ complete contract research needs for in vitro studies to complement our in vivo models, Perry Scientific offers a variety of standardized cell culture assays in our In Vitro Testing Lab.

Our scientists have applied decades of industrial experience in drug development and cosmetics testing to the development of standardized in-house procedures with a wide variety of human and animal cell types and cell culture systems.

From monolayers of primary human cells, to organotypic 3D human skin models with tissue-like properties, to alternative non-animal models such as the CAMVA model, we have the expertise you need for basic R&D studies, all the way through product efficacy and claims testing.

An area of focus is in skin and dermatology. We offer particular expertise in studies of the extracellular matrix, with a monolayer high-throughput assay that measures collagen deposition and cytoxicity in primary human dermal fibroblasts, to our proprietary all-human, 3D tissue-engineered dermal equivalent model.

We can also perform topical testing with a variety of commercially-available full-thickness skin equivalents, utilizing proprietary flow cytometry assays for ECM expression, antioxidant effects, and epidermal differentiation.

Areas of Expertise

• Skin and eye irritation
• Cell viability and toxicity
• Skin protection
• Collagen synthesis
• Apoptosis
• Cell proliferation
• Protein expression
• UV protection
• Penetration
• Cell renewal
• Antioxidant activity

Methods and Techniques

• Cell and molecular biology
• Histology
• Immunofluorescence
• Skin thickness
• Image analysis
• ELISA
• FACS
• Cytotoxicity and hemolysis
• PCR

Tools and Studies

• Primary cells and cell line cultures
• Human Skin Equivalent Cultures
• Reconstituted skin
• Explants/biopsies from various species
• HET-CAM
• CAMVA
• Collagen Deposition
• Metabolic Studies